Sample comparisons

Wild-type vs. golden (gol) mutant.

wild-type vs golden

Top: Adult wild-type (Connor)

Bottom: Adult golden (gol) mutant.

Wild-type vs. Huli hutu (hht) mutant

wild-type vs huli hutu comparison

Mutant image (bottom) is of a 7dpf huli hutu (hht), ENU-generated mutation currently being fine mapped (in-house). Normal image (top) is of a comparable 7dpf wild-type.

  • hht causes striking architectural and cytologic changes in several organs
  • The eyes of a variable fraction of mutants are reduced in size
  • Cell types of different retinal layers are mixed together to varying degrees
  • The eye phenotype is detectable at 2dpf and can be mild to severe at 7dpf
  • Severe cytologic atypia is seen in the intestine, pancreas, liver, swim bladder, and pneumatic duct
  • This atypia includes a great variability in nuclear size and shape, frequent multinucleation, and architectural disorganization

from Mohideen, et al "Histology-based screen for zebrafish mutants with abnormal cell differentiation" in Dev Dyn 228:414-423 2003.

H&E stain vs. MicroCT

histology vs microCT comparison

3-Way Comparison of 60 dpf in MicroCT

microct 3-way comparison

Top: A screen-capture of part of a 3D model generated from these 1500 2D slices in a Volume Graphics software package known as VGStudio Max.

Middle: One of 1500 digital, coronal 2D slices generated by applying the Feldkamp cone-beam reconstruction algorithm to these 600 x-rays.

Bottom: One of 600 x-rays taken through 180° of the head of an unstained, 60 dpf juvenile zebrafish (one x-ray every 0.3°).

Normal vs. Tumor

normal vs tumor comparison

Left: a healthy specimen

Right: a specimen bearing a tumor on the right side of the section. Age unknown but older than the other fish.