Wild-type vs. golden (gol) mutant.
Top: Adult wild-type (Connor)
Bottom: Adult golden (gol) mutant.
Wild-type vs. Huli hutu (hht) mutant
Mutant image (bottom) is of a 7dpf huli hutu (hht), ENU-generated mutation currently being fine mapped (in-house). Normal image (top) is of a comparable 7dpf wild-type.
- hht causes striking architectural and cytologic changes in several organs
- The eyes of a variable fraction of mutants are reduced in size
- Cell types of different retinal layers are mixed together to varying degrees
- The eye phenotype is detectable at 2dpf and can be mild to severe at 7dpf
- Severe cytologic atypia is seen in the intestine, pancreas, liver, swim bladder, and pneumatic duct
- This atypia includes a great variability in nuclear size and shape, frequent multinucleation, and architectural disorganization
from Mohideen, et al "Histology-based screen for zebrafish mutants with abnormal cell differentiation" in Dev Dyn 228:414-423 2003.
H&E stain vs. MicroCT
3-Way Comparison of 60 dpf in MicroCT
Top: A screen-capture of part of a 3D model generated from these 1500 2D slices in a Volume Graphics software package known as VGStudio Max.
Middle: One of 1500 digital, coronal 2D slices generated by applying the Feldkamp cone-beam reconstruction algorithm to these 600 x-rays.
Bottom: One of 600 x-rays taken through 180° of the head of an unstained, 60 dpf juvenile zebrafish (one x-ray every 0.3°).
Normal vs. Tumor
Left: a healthy specimen
Right: a specimen bearing a tumor on the right side of the section. Age unknown but older than the other fish.